CCCadvanced® FN1 motifs Cell Culture Flasks

New
CCCadvanced® FN1 motifs Cell Culture Flasks
New
CCCadvanced® FN1 motifs Cell Culture Flasks
New
CCCadvanced® FN1 motifs Cell Culture Flasks
New
CCCadvanced® FN1 motifs Cell Culture Flasks
New
CCCadvanced® FN1 motifs Cell Culture Flasks
CCCadvanced® FN1 motifs Cell Culture Flasks
CCCadvanced® FN1 motifs Cell Culture Flasks
CCCadvanced® FN1 motifs Cell Culture Flasks
CCCadvanced® FN1 motifs Cell Culture Flasks
CCCadvanced® FN1 motifs Cell Culture Flasks

Cultivating iPSCs, MSCs, primary cells or other ECM-dependent cells? Tired of lot-to-lot coating media variation/validation, tedious preparation and increased contamination risk? How about a defined synthetic, xeno-free surface for stable long-term expansion of your stem cells?

CCCadvanced® FN1 motifs cell culture flasks are ready-to-use. They can be also used under restrictive culture conditions (serum- and xeno-free media). More information

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Product Information

CCCadvanced FN1 motifs consumables are classical Eppendorf Cell Culture Consumables, additionally coated with a defined synthetic animal- and human-component-free substrate. The substrate (fibronectin-derived motifs with optimized steric configuration) is designed to mimic the cell attachment site of native ECM.

Learn more about cultivation of human iPSCs and MSCs on FN1 motifs and smart features of our cultureware below.

iPSCs: Efficient long-term expansion in a completely synthetic culture system



› Supports efficient long-term hiPSC expansion in a completely defined, animal-, and human-component-free culture system for 25 passages
› Consistent and robust growth rate
› Typical morphology remains stable (Fig. 1)
› hiPSCs remain undifferentiated and maintain functional pluripotency
› Maintenance of trilineage differentiation potential after long-term expansion while exhibiting normal genomic integrity
› For further details see Application Note 389 under “Notes & Papers” below

CCCadvanced® FN1 motifs Cell Culture Flasks

MSCs: Animal-component-free expansion of human mesenchymal stem cells



› Supports efficient hMSC proliferation in a completely animal-component-free environment even on long term (up to 10 passages)
› Characteristic morphology (Fig. 2) remains stable for 10 successive passages without signs of replicative senescence
› Stable and robust proliferation rate
› Validated with hMSC from different tissue origins
› Undifferentiated hMSCs retain their multi-lineage differentiation potential after expansion
› For further details see Application Note 390 under “Notes & Papers” below

CCCadvanced® FN1 motifs Cell Culture Flasks

Cell accessibility


A unique ConvexAccess™ neck geometry significantly facilitates the access to the growth area and makes cell seeding and media exchange more convenient, safe and reliable

CCCadvanced® FN1 motifs Cell Culture Flasks

Improved contamination protection


The new hydrophobic volume filter pad is more efficient than standard filter membranes

CCCadvanced® FN1 motifs Cell Culture Flasks

Corrugated cap


Anti–rolling cap with corrugation and clear position marks in plug–seal caps to prevent undesired full–closing

CCCadvanced® FN1 motifs Cell Culture Flasks

Features

  • Suitable for iPSCs, MSCs, primary cells, and other ECM-dependent cells
  • Ready-to-use: no tedious preparation with possible vessel/coating media dissipation
  • Synthetic surface: coated with synthetic fibronectin-derived motifs (including RGD peptide sequence, optimized steric configuration to mimic ECM proteins)
  • Xeno-free materials: manufactured by using animal- and human-component-free materials
  • High experimental flexibility: suitable for many cell types, dissociation media (e.g., Accutase®, TrypLE™ reagents), as well as serum-, animal-, and human-component-free media (see list in Download section)
  • Easy logistics: shelf life of 36 months from date of manufacture (stored dry at 15 °C to 30 °C)
  • Lot-to-lot production consistency: no expensive lot-specific performance verification of coating media
  • Reduced contamination risk: no preparation needed, individually packed, and several smart features for safer handling
  • High-quality base material: polystyrene (meets requirements of USP Class VI). Manufactured with Quality Management System (QMS) compliant to standard QMS (e.g., ISO 13485).
  • Compatible with cell-based assays and staining procedures: no autofluorescence

Applications

  • Expansion and differentiation of stem cells (e.g., hiPSCs, hMSCs)
  • Differentiation of primary cells and other ECM-dependent eukaryotic cells
  • Feeder-free cell culture
  • Restrictive culture conditions (serum- and xeno-free)
 FN1 motifs T-75 flaskFN1 motifs T-175 flask
Materialpolystyrene, meets requirements of USP Class VIpolystyrene, meets requirements of USP Class VI
Operating temperature15 °C – 37 °C15 °C – 37 °C
Packing5 flasks, individually wrapped5 flasks, individually wrapped
Purity grade(s)Sterile, pyrogen-, DNase-, RNase-, human and bacterial DNA-free. Non-cytotoxicSterile, pyrogen-, DNase-, RNase-, human and bacterial DNA-free. Non-cytotoxic
SurfaceCoated with synthetic fibronectin-derived motifsCoated with synthetic fibronectin-derived motifs
Xeno-freeManufactured using animal- and human-component-free materialsManufactured using animal- and human-component-free materials
Storage temperatureStore dry at room temperature from 15 °C to 30 °CStore dry at room temperature from 15 °C to 30 °C
Growth area/well177,9 mm²179,5 mm²
Theoretical total volume279,8 mL662,1 mL
Working volume8.0 – 20.0 mL20.0 – 30.0 mL
CentrifugationCan be centrifuged with the suitable adapters; refer to the manufacturer’s instructionsCan be centrifuged with the suitable adapters; refer to the manufacturer’s instructions
DimensionsConforms to ANSI/SLAS 1-2004: Microplates – Footprint dimensionsConforms to ANSI/SLAS 1-2004: Microplates – Footprint dimensions
1For the initial seeding of cells, take into account the growth area in order to support optimal cell growth.

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