BioBLU® f Single-Use Vessels

Image – BioBLU 3f with medium, front view
Image – BioBLU f
Image – BioBLU 3f
Image – BioBLU 3f Head Plate
Image – BioBLU 0.3f new design, without medium
BioBLU® f Single-Use Vessels
Image – BioBLU 3f with medium, front view
Image – BioBLU f
Image – BioBLU 3f
Image – BioBLU 3f Head Plate
Image – BioBLU 0.3f new design, without medium
BioBLU® f Single-Use Vessels
BioBLU® f Single-Use Vessels

BioBLU f Single-Use Vessels are specifically designed for robust microbial applications using bacteria, yeasts, and fungi allowing microbial process development at the highest level. The rigid wall stirred tank design mimicks traditional vessels and supports effective mixing and mass transfer making them suitable for high-cell density fermentation. More information

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Product Information

Eppendorf BioBLU Single-Use Vessels combine all the advantages of single-use technology with the trusted performance and scalability of a stirred-tank design. Single-use vessels eliminate labor-intensive cleaning, improve turn-around time, simplify validation, and reduce overall costs. BioBLU f vessels are specifically designed for robust microbial applications using bacteria, yeasts, and fungi.

Features

  • Single-use, stirred-tank, rigid-walled vessels available in sizes ranging from 65 mL to 3.75 L working volume
  • Designed for high-density fermentation processes
  • Multiple Rushton-type impellers for efficient mixing and mass transfer
  • Sealed magnetic drive with fully enclosed bearings maintain vessel sterility
  • Baffles for excellent mixing and mass transfer
  • Vessels are assembled with sparger, gas filters for inlet and exhaust as well as penetrations for pH, DO, temperature, liquid additions, sampling, and harvest
  • For use with Eppendorf benchtop bioreactor systems
  • Adaptor kits for legacy bioreactor controllers available
  • Warranty: 6 Months Parts and Labour

Applications

  • Bacteria, yeasts, and fungi
  • Suitable for high-cell density fermentation
 BioBLU® 0.3fBioBLU® 1fBioBLU® 1fBioBLU® 3f
Bioreactors    
Head plate ports2x Pg 13.5, 1x harvest tube, 1x thermowell, 1x sample port, 2x liquid addition overlay, 1x liquid addition submerged, 1x DO sensor port with permeable membrane, 1x gas sparge, 1x exhaust3x Pg 13.5, 1x harvest tube,1x thermowell, 1x sample port, 3x liquid addition overlay, 2x liquid addition submerged, 1x DO sensor port with permeable membrane, 1x gas sparge, 1x exhaust, 4x baffles (incl. cooling water connection)3x Pg 13.5, 1x harvest tube,1x thermowell, 1x sample port, 3x liquid addition overlay, 2x liquid addition submerged, 1x DO sensor port with permeable membrane, 1x gas sparge, 1x exhaust, 4x baffles (incl. cooling water connection)4x Pg 13.5, 1x harvest tube,1x thermowell, 1x sample port, 3x liquid addition overlay, 1x liquid addition submerged, 1x DO sensor port with permeable membrane, 1x gas sparge, 1x exhaust, 4x baffles (incl. cooling water connection)
Material tubingSiliconeSiliconeSiliconeSilicone
Material vessel1PS, PC (USP Class VI)PS, PC (USP Class VI)PS, PC (USP Class VI)PC (USP Class VI)
SterilizationPre-sterilized (15 kGy β-irradiated)Pre-sterilized (15 kGy β-irradiated)Pre-sterilized (15 kGy β-irradiated)Autoclavable, no pre-sterilization
Working volume (total)65 – 250 mL (380 mL)250 mL – 1.25 L (1.8 L)250 mL – 1.25 L (1.8 L)1.25 L – 3.75  L (5 L)
Agitation    
DriveMagnetic overhead drivesMagnetic overhead drivesMagnetic overhead drivesMagnetic overhead drives
ImpellersRushton-typeRushton-typeRushton-typeRushton-type
Recommended agitation speed20 – 2,000 rpm100 – 1,500 rpm100 – 1,500 rpm25 – 1,200 rpm
Gassing    
Gas supplySpargerSpargerSpargerSparger
Monitoring and Control    
DO SensorPolarographic DO - 4.7/118 mmPolarographic DO - 4.7/229 mmPolarographic DO - 4.7/229 mmPolarographic DO - 12/220 mm
Exhaust condensationLiquid-free cooling (Peltier)Liquid-free cooling (Peltier)/water-cooledLiquid-free cooling (Peltier)/water-cooledWater-cooled
Max. operating temperature45 °C45 °C45 °C45 °C
Sensor length120 mm220 mm220 mm220 mm
Temperature sensorPt100 RTD (not included)Pt100 RTD (not included)Pt100 RTD (not included)Pt100 RTD (not included)
pH sensorStandard glass sensor (120 mm)Standard glass sensor (220 mm)Standard glass sensor (220 mm)Standard glass sensor (225 mm)
1PC = Polycarbonate, PS = Polystyrene
BioBLU® f Single-Use Vessels
Video: BioBLU® f Single-Use Vessels – Video – BioBLU Vessel Setup